BioSep Acoustic Perfusion System
Retention system for perfusion, concentration and washing
The Applikon BioSep system is a unique, cell retention device for high-density perfusion processes. Using high frequency resonant ultrasonic waves to separate cells instead of a physical mesh or membrane, it offers all the benefits of traditional devices but without their inherent problems and limitations.
The BioSep, based on the technology of acoustic resonance, is a non fouling / non clogging retention system. It can be applied in continuous mode which makes the BioSep the most favourable instrument for perfusion cultures. The BioSep can be applied in both R&D (max. 1L/day), process development and on production scale (1000L/dag). Screening of cells and medium optimalisation typically are applications in the R&D segment. Furhtermore the BioSep can be seen as the first step in the DownStream Processing. The economic advantages are evident and described in literature.The technology of the BioSep also is very attractive for applications such as concentration of cell / particles and washing (e.g. beads).
Acoustic perfusion process
An acoustic perfusion process using the BioSep typically involves continuous addition of fresh medium to the bioreactor, while cells are filtered from the harvest stream by the resonator chamber and returned to the bioreactor. The BioSep can be directly mounted onto the bioreactor head plate. One standard mode of operation employs, for example, a harvest pump at the exit port of the resonator chamber, and a recirculation pump for the return of separated cells that settled from the acoustic energy field within the resonator chamber. Alternatively the BioSep system can also be set up to allow for semicontinuous operation or alternative strategies of cell recirculation.
Acoustic perfusion is generally applicable for suspended mammalian and animal cell culture, but can also be adopted for anchorage dependent cell lines, or the perfused culture of plant cells. The BioSep separation principle is purely based on gentle, acoustically induced loose aggregation followed by sedimentation. In contrast to other cell separation techniques, the acoustic energy mesh created within the BioSep constitutes “virtual”, thus superior-, non-contact, non-fouling, non-moving filtration means allowing for up to thousands of hours of continuous operation. As a result, greatly increased steady state cell density, productivity, and product quality is obtained.
The Biosep comes in different versions ranging from a capacity of 1L upto 1000L/day, divided over five different models. BioSep is using SonoSep Technology.
All systems can be used in re-circulation mode and in single pass mode.
BioSep is the ultimate tool for cell culture perfusion from 100 ml scale bioreactor up to production scale.
BioSep is the only system available in the market that cover the complete range of cultivation volumes. The no-fouling and no-blocking technology can be used for extended periods and requires a minimum maintenance since no movig parts are used to separate cells from the culture medium.
Other applications for the BioSep:
- cell concentration
- cell washing
Features, Advantages and Benefits:
- Proven under cGMP conditions
- Cost effective
- Easy to install and to operate
- Applicable on any type and brand of bioreactor
- long term cultivation possible (>60 days)
- no fouling or blocking
- no damage to the cells
- increased cell viability
- high separation efficiency