The stirred tank reactor (STR) is the most widely used bioreactor type. Applikon offers a unique product, the Bio Clave, as an autoclavable stainless steel bioreactor for cell culture and microbial culture applications.

The Bio Clave bioreactors are versatile, easy to use systems that offer economical alternatives to steam-in-place bioreactors and fermentors The Bio Clave range combines the advantages of both the steam-in place bioreactor (robust design and pressurizable) and the glass autoclavable bioreactors (flexibility and modularity). The 316L stainless steel vessels are constructed according to the latest standard on hygienic processing and cGMP. The interior finish of the vessel is electropolished to Ra=0.4µm, while the exterior is mirror polished. The high-grade surface finish secures a better cleanability. The systems are built according to the specific demands of a process using an extensive array of standard components. Because of the modularity and flexibility, the user can always adapt the systems to changed process demands. This results in low initial investment and low running costs.

The Bio Clave bioreactor and fermentor are available in 20, 70 and 110 liter total volume. All systems are equipped with a top driven stirrer who can be either a magnetically coupled or a lipsealed stirrer system.

Bioreactors and fermentors
Applikon Bio Clave bioreactor and fermentor systems are designed for maximum flexibility in setting up. The reactor headplate only has entry ports and no welded pipes. This means that the systems can be completely customized for the user's application. When the application changes, the bioreactor set-up can easily be adapted to the changed process demands. Headplates are made of 316L stainless steel and all parts in the bioreactors have an electropolished finish that ensures reliable cleanability of the vessel and components. Vessels can be used up to 3.1 barg of overpressure.

Lipseal or Magnetic Stirrers
Ports:
- Head plate: 1x69.2mm,3x1.5"TC,4x27 & 3x16mm
- Upper Side Wall: 5x27mm - Lower Side Wall: 5xDN25

Many Auxiliaries

	Bio Clave 20L	Bio Clave 70L	Bio Clave 110L
Bio Clave reactor type	20 liter	70 liter	110 liter
Inner Diameter	211 mm	316 mm	370 mm
Inner Height (max.)	631 mm	957 mm	1043 mm
Liquid Height (working vol.)	444 mm	678 mm	764 mm
Ports Head Space section	5 x 27 mm	5 x 27 mm	5 x 27 mm
Ports Bottom section	5 x DN 25	5 x DN 25	5 x DN 25
Ports Head Plate:
	1 x 69 mm	1 x 69 mm	1 x 69 mm
	3 x 16 mm	3 x 16 mm	3 x 16 mm
	4 x 27 mm	4 x 27 mm	4 x 27 mm
	3 x 1.5"TC	3 x 1.5"TC	3 x 1.5"TC
Autoclave Space (HxD)	1200 x 400 mm	1900 x 600 mm	1985 x 650 mm
Overall height reactor	715 mm	1022 mm	1110 mm
Total Volume	20 liter	70 liter	110 liter
Working Volume	15 liter	50 liter	80 liter
Min. Working Volume	4 liter	13 liter	21 liter
H/D Total	3	3	3
H-D Work Vol.	2.2	2.2	2.2
Design Pressure Reactor	4.1 bar	4.1 bar	4.1 bar
Test Pressure Reactor	4.8 bar	4.8 bar	4.8 bar
Design Pressure Jacket	6.2 bar	6.2 bar	6.2 bar
Test Pressure Jacket	11.2 bar	11.2 bar	11.2 bar
Max. Temperature Reactor	130 °C	130 °C	130 °C